Surface plasmon resonance

نویسنده

  • P. Anton van der Merwe
چکیده

4. LIGAND 10 4.1. Direct versus indirect immobilisation 10 4.2. Covalent immobilisation 11 4.2.1. A general approach 11 4.2.2. Choice of chemistry 12 4.2.3. Prepare the protein 12 4.2.4. Pre-concentration 12 4.2.5. Amine coupling 14 4.2.6. Regeneration 15 4.2.7. Adjusting the immobilisation conditions. 16 4.3. Non-covalent immobilisation (ligand capture) 16 4.3.1. Using an existing strategy 17 4.3.2. Developing a new strategy 17 4.4. Activity of immobilised ligand 18 4.5. Control surfaces 19 4.6. Re-using sensor chips 19

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تاریخ انتشار 2003